Inventory of some Tulipa species from Uzbekistan using DNA barcoding

Climate fluctuations in the Quaternary period significantly influenced evolution and distribution of plants, which caused to a serious genetic effects speciation activity [1]. Thus, Central Asia is distinguished by wide variety flora, including 9520 species, while 20% are endemics [2]. Great phenotypic diversity wild flora Uzbekistan, numbering about 4400 species vascular plants determined peculiar climatic conditions, zoning, altitudinal zones [3]. At same time, being one centers early diversification monocotyledonous geophytes, taxonomically complex representatives this group not sufficiently involved phylogenetic studies. This study pilot Uzbekistan using DNA barcoding method, that based on sequencing phylogenetically significant sequences. Four plastid (rbcL, psbA-trnH, matK, trnL-F) nuclear (ITS) markers were used for identification genus Tulipa. The results molecular analysis 15 Tulipa (Liliaceae) marker sequence encoding ITS fragment presented. successful usefulness technology inventory has been demonstrated.